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Annual Review of Biomedical Engineering

Volume 26, 2024

Direct Electron Transfer–Type Oxidoreductases for Biomedical Applications

Abstract

Among the various types of enzyme-based biosensors, sensors utilizing enzymes capable of direct electron transfer (DET) are recognized as the most ideal. However, only a limited number of redox enzymes are capable of DET with electrodes, that is, dehydrogenases harboring a subunit or domain that functions specifically to accept electrons from the redox cofactor of the catalytic site and transfer the electrons to the external electron acceptor. Such subunits or domains act as built-in mediators for electron transfer between enzymes and electrodes; consequently, such enzymes enable direct electron transfer to electrodes and are designated as DET-type enzymes. DET-type enzymes fall into several categories, including redox cofactors of catalytic reactions, built-in mediators for DET with electrodes and by their protein hierarchic structures, DET-type oxidoreductases with oligomeric structures harboring electron transfer subunits, and monomeric DET-type oxidoreductases harboring electron transfer domains. In this review, we cover the science of DET-type oxidoreductases and their biomedical applications. First, we introduce the structural biology and current understanding of DET-type enzyme reactions. Next, we describe recent technological developments based on DET-type enzymes for biomedical applications, such as biosensors and biochemical energy harvesting for self-powered medical devices. Finally, after discussing how to further engineer and create DET-type enzymes, we address the future prospects for DET-type enzymes in biomedical engineering.

Keyword(s): biosensors, continuous monitoring, DET-type enzyme, direct electron transfer, energy harvesting, fructose dehydrogenase, glucose dehydrogenase
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/content/journals/10.1146/annurev-bioeng-110222-101926
2024-07-03
2024-07-04
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/content/journals/10.1146/annurev-bioeng-110222-101926
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Direct Electron Transfer–Type Oxidoreductases for Biomedical Applications
Annual Review of Biomedical Engineering 26, 357 (2024); https://doi.org/10.1146/annurev-bioeng-110222-101926
/content/journals/10.1146/annurev-bioeng-110222-101926
/content/journals/10.1146/annurev-bioeng-110222-101926
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  • Article Type: Review Article

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