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Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 15
A video from the 2014 review by Hai Qi Wolfgang Kastenmüller and Ronald N. Germain "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue" from the Annual Review of Cell and Developmental Biology.
Shown: An isotropic ICOS-triggering field promotes coordinated actin dynamics pseudopod extension and persistent motility of T cells
After invitro activation and retroviral transduction with the LifeAct-mRubby F-actin reporter T cells were imaged by TIRF microscopy on the anti-ICOS antibody-coated lipid bilayer.
Original publication: Xu H Li X Liu D Li J Zhang X et al. 2013. Follicular T-helper cell recruitment governed by bystander B cells and ICOS-driven motility. Nature 496:523–27
Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 16
A video from the 2014 review by Hai Qi Wolfgang Kastenmüller and Ronald N. Germain "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue" from the Annual Review of Cell and Developmental Biology.
Shown: Icos −/− T cells are impaired in pseudopod dynamics and persistent motility at the T-B border in the lymph node
GFP-expressing Icos +/+ (green) and dsRed-expressing Icos −/− (red) OT-2 T cells were imaged at the same T-B border. The image sequence was played twice (version a and b). (a) Regular 20 frames per second replay. (b) The depolarized state of both Icos +/+ (4 out of 81 ~5%) and Icos −/− T cells (12 out of 51 ~24%) is marked on six equally spaced time frames exactly as used in the quantitation procedure described in the method.
Original publication: Xu H Li X Liu D Li J Zhang X et al. 2013. Follicular T-helper cell recruitment governed by bystander B cells and ICOS-driven motility. Nature 496:523–27
Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 17
A video from the 2014 review by Hai Qi Wolfgang Kastenmüller and Ronald N. Germain "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue" from the Annual Review of Cell and Developmental Biology.
Shown: Sap −/− T cells fail to be recruited and retained in nascent GC
CFP-expressing Sap +/+ OT-2 T cells (red) GFP-expressing Sap −/− OT-2 T cells (green) and non-fluorescent MD4 B cells were cotransferred into mice that were subsequently immunized with HEL-OVA. Intravital imaging of draining LNs was performed six to eight days post immunization. CMTPX-labeled polyclonal naïve B cells (blue) were transferred one or two days prior to imaging to help demarcate follicular mantle and GC. The dotted line at the beginning of the video approximates the GC border in this maximum intensity projection.
Original publication: Qi H Cannons JL Klauschen F Schwartzberg PL Germain RN. 2008. SAP-controlled T-B cell interactions underlie germinal centre formation. Nature 455:764–69
Viral Manipulation of Plant Host Membranes: Video 2
A video from the 2014 review by Jean-François Laliberté and Huanquan Zheng "Viral Manipulation of Plant Host Membranes" from the Annual Review of Virology.
A Turnip mosaic virus (TuMV)-induced 6K2-tagged vesicle moves intercellularly. The video shows a photoactivated cell infected with TuMV expressing 6K2:PAGFP. Images were acquired every 3 s using the 40× objective of a LSM 780 confocal microscope. Reproduced with permission from Grangeon et al. Front. Microbiol. 4:351 (2013).
Three-Dimensional Imaging of Viral Infections
Introduction to the article "Three-Dimensional Imaging of Viral Infections” written by Cristina Risco Isabel Fernández de Castro and Laura Sanz-Sánchez of the National Center for Biotechnology in Madrid as well as Kedar Narayan Giovanna Grandinetti and Sriram Subramaniam of the National Cancer Institute in the United States.
Return to the Annual Review of Virology Video Table of Contents.
PHIRE and TWiV: Experiences in Bringing Virology to New Audiences
Graham F. Hatfull of the University of Pittsburgh introduces his article for the 2014 Annual Review of Virology titled "PHIRE and TWiV: Experiences in Bringing Virology to New Audiences."
Return to the Annual Review of Virology Video Table of Contents.
The Peculiarities and Paradoxes of Plasmodium Heme Metabolism: Supplemental Video 1
A supplemental video from the 2014 review by Paul A. Sigala and Daniel E. Goldberg "The Peculiarities and Paradoxes of Plasmodium Heme Metabolism" from the Annual Review of Microbiology.
Shown: In their native environment within live parasites hemozoin crystals are frequently observed to be dynamically moving within the digestive vacuole.
Viruses and the Microbiota
Julie K. Pfeiffer of the Department of Microbiology University of Texas Southwestern Medical Center introduces her article for the 2014 Annual Review of Virology titled "Viruses and the Microbiota."
Return to the Annual Review of Virology Video Table of Contents.
Live Cell Imaging of Retroviral Entry: Video 1
A video from the 2014 review by Amy E. Hulme and Thomas J. Hope "Live Cell Imaging of Retroviral Entry" from the Annual Review of Virology.
Shown: Detection of viral fusion with live cell imaging. The fusion of Tomato-Vpr/iGFP-labeled HIV-1 with CHO cells was imaged at 37°C under a blood/gas mixture of CO2 on a DeltaVision OMX microscope. The experiment was imaged for 44 min with a Z series of images taken every 45 s. Viral fusion was observed as a decrease in GFP fluorescence after 37.5 min due to release of the fluid phase GFP marker into the cytoplasm of the cell. The Tomato-Vpr-labeled virus retained a lower amount of GFP fluorescence after fusion due to GFP localization to the interior of the capsid (Figure 1). See Figure 2 for further details.
Glycan Engagement by Viruses: Receptor Switches and Specificity
Thilo Stehle of Interfaculty Institute for Biochemistry University of Tübingen introduces his article for the 2014 Annual Review of Virology titled "Glycan Engagement by Viruses: Receptor Switches and Specificity."
Return to the Annual Review of Virology Video Table of Contents.
Oncolytic Poxviruses
Winnie M. Chan and Grant McFadden of the Department of Molecular Genetics and Microbiology University of Florida introduce their article for the 2014 Annual Review of Virology titled "Oncolytic Poxviruses."
Return to the Annual Review of Virology Video Table of Contents.
Introducing the Annual Review of Virology
In this presentation Annual Review of Virology Editor Lynn Enquist and Associate Editors Terence S. Dermody and Daniel DiMaio discuss what motivated them to launch the journal. They explain how they appointed editorial committee members and how they work together to select contributing authors and article topics for each volume. They underscore the need to keep up with the discoveries and technological advances in virology while maintaining the focus on the future of the field. In this mission they endeavor to engage a broad audience keeping the articles clear so as to serve as support for both teaching and research.
Lynn Enquist is Henry L. Hillman Professor at the Department of Molecular Biology and Princeton Neuroscience Institute of Princeton University.
Terence S. Dermody is Dorothy Overall Wells Professor of Pediatrics and Director of the Division of Pediatric Infectious Disease at the Vanderbilt University School of Medicine.
Daniel DiMaio is Waldemar Von Zedtwitz Professor of Genetics and Professor of Molecular Biophysics and Biochemistry at the Department of Genetics of the Yale University School of Medicine.
Return to the Annual Review of Virology Video Table of Contents.
Viral Manipulation of Plant Host Membranes: Introduction
Jean-François Laliberté of Institut National de la Recherche Scientifique Institut Armand-Frappier introduces his article for the 2014 Annual Review of Virology titled "Viral Manipulation of Plant Host Membranes."
Return to the Annual Review of Virology Video Table of Contents.
Cytoplasmic RNA Granules and Viral Infection: Video 1
A video from the 2014 review by Wei-Chih Tsai and Richard E. Lloyd "Cytoplasmic RNA Granules and Viral Infection" from the Annual Review of Virology.
Shown: Virus-induced stress granules (small bright green foci) forming in cells infected with coxsackievirus B3 that produces dsRed fluorescent protein as a marker of robust replication. HEK cells are expressing Tia1-GFP (green) as a marker for stress granules which translocates from the nucleus to cytoplasm as infection initiates. Expression of Tia1-GFP makes the cells more prone to stress granule formation which generally delays or inhibits coxsackievirus B3 replication. In other infected cells stress granules shrink or disperse after they appear or they persist as residual pseudo–stress granules that no longer contain stalled translation complexes.
Viral Manipulation of Plant Host Membranes: Video 1
A video from the 2014 review by Jean-François Laliberté and Huanquan Zheng "Viral Manipulation of Plant Host Membranes" from the Annual Review of Virology.
Three-dimensional confocal microscopy rendering of a leaf epidermal cell from Nicotiana benthamiana that is expressing the GFP-HDEL endoplasmic reticulum marker and has been infected with TuMV producing 6K2:mCherry. The images in the video are three-dimensional renderings of >40 1-μm-thick slices that overlap by 0.5 μm. Reproduced with permission from Grangeon et al. J. Virol. 86:9255--65 (2012).
Three-Dimensional Imaging of Viral Infections: Video 1
Shown: 3D model of the reovirus inclusion assembled in a HeLa cell infected under permissive conditions (Figure 1a). Reproduced with permission from Reference 35.
Three-Dimensional Imaging of Viral Infections: Video 2
A video from the 2014 review by Cristina Risco Isabel Fernández de Castro Laura Sanz-Sánchez Kedar Narayan Giovanna Grandinetti and Sriram Subramaniam "Three-Dimensional Imaging of Viral Infections" from the Annual Review of Virology.
Shown: 3D model of the reovirus inclusion assembled in an MDCK cell infected under nonpermissive conditions. Reproduced with permission from Reference 35.
Three-Dimensional Imaging of Viral Infections: Video 3
A video from the 2014 review by Cristina Risco Isabel Fernández de Castro Laura Sanz-Sánchez Kedar Narayan Giovanna Grandinetti and Sriram Subramaniam "Three-Dimensional Imaging of Viral Infections" from the Annual Review of Virology.
Shown: 3D model of a whole Huh7.5 cell infected with the hepatitis C virus (HCV). Reproduced with permission from Reference 33.
Three-Dimensional Imaging of Viral Infections: Video 4
A video from the 2014 review by Cristina Risco Isabel Fernández de Castro Laura Sanz-Sánchez Kedar Narayan Giovanna Grandinetti and Sriram Subramaniam "Three-Dimensional Imaging of Viral Infections" from the Annual Review of Virology.
Shown: Animation showing the membranous network assembled by severe acute respiratory syndrome (SARS) coronavirus in Vero E6 cells. Reproduced with permission from Reference 59.
Three-Dimensional Imaging of Viral Infections: Video 5
A video from the 2014 review by Cristina Risco Isabel Fernández de Castro Laura Sanz-Sánchez Kedar Narayan Giovanna Grandinetti and Sriram Subramaniam "Three-Dimensional Imaging of Viral Infections" from the Annual Review of Virology.
Shown: Electron tomography and 3D modeling of early coxsackievirus B3–induced membrane structures in Vero E6 cells. Reproduced with permission from Reference 60.