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Cytoplasmic Streaming in the Drosophila Oocyte: Video 5
A video from the 2016 review by Margot E. Quinlan "Cytoplasmic Streaming in the Drosophila Oocyte" from the Annual Review of Cell and Developmental Biology.
Shown: The actin mesh in a stage 9 Drosophila oocyte is labeled by UtrnCH-GFP (the actin binding calponin homology domain of utrophin which labels filamentous actin). Images were acquired every 10 seconds.
Crafting a Manuscript: It's Easier to Take Your Voice Out Than to Get It Back In
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Are We Getting Further Away from Organizations?
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Research Ideas: Where to Look for Inspiration
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Having a Broader Influence on the Field: Why Being an Editor Matters
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
For Love of Basketball: How to Stay Engaged and Motivated
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Do You Need a Program of Research?
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Successful Scholars Also Publish: Developing a Portfolio of Research
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Where Do We Discover New Constructs?
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Go to Where the Action Is: Why We Need More Experience In and With Organizations
The Inspiration Behind Escalation of Commitment Research
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Beyond Hammers and Nails: Toward a Social Psychology of Organizations
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
The Dominance of Publishing in Top-Tier Journals: A Tale of Caution
Barry M. Staw Professor Emeritus at the Haas School of Business talks about his life and career with Frederick P. Morgeson Eli Broad Professor of Management at the Eli Broad College of Business at Michigan State University.
Moving On Out: Transport and Packaging of Influenza Viral RNA into Virions: Video 1
A video from the 2016 review by Seema S. Lakdawala Ervin Fodor and Kanta Subbarao "Moving On Out: Transport and Packaging of Influenza Viral RNA into Virions" from the Annual Review of Virology.
Shown: Inverted selective plane illumination microscopy video of Madin-Darby canine kidney (MDCK) cells infected with influenza A/WSN/33 PA-green fluorescent protein (GFP). MDCK cells were infected for 16 h and imaged for 30 min with an entire cell volume captured every 2 s. Scale bar = 10 µm. Video reproduced with permission from Reference 10.
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 1
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: Cell lysis following λ infection as seen under phase-contrast illumination (see also Figure 2e). The cell is infected at t = 0 and lyses at t ≈ 80 min. Video courtesy of Louis McLane and Samuel Skinner; Golding lab (unpublished).
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 2
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: Tracking the postinfection decision (see also Figure 2d ). Phage capsids were labeled using gpD-YFP. At t = 0 two cells are each infected by a single phage (green) and one cell is infected by three phages. The two cells infected by single phages go into the lytic pathway as indicated by the intracellular production of new phage capsids (green). The cell infected by three phages goes into the lysogenic pathway as indicated by the activity of a PRE-mCherry reporter (red). Eventually the lytic pathway results in cell lysis whereas the lysogenic cell continues to grow and divide. Video reproduced from Zeng et al. 2010 Cell 141:682–91 with permission from Elsevier.
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 3
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: PR activity during lysogen induction (see also Figure 2g ). In the reporter strain mRNA from the PR promoter is labeled using MS2-GFP (green) whereas the genome locus where the reporter resides is labeled using TetR-mCherry (red). The cells are λ lysogens. At t = 20 min mitomycin C is added leading to transient derepression of PR and induction of the dormant prophage. The prophage used is lysis deficient; hence the cells do not lyse at the end of the induction process. Video courtesy of Jing Zhang Louis McLane and Samuel Skinner; Golding lab (unpublished).
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 4
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: Single-molecule imaging of CI production (yellow) during stable lysogeny. Video reproduced from Hensel et al. 2012 Nat. Struct. Mol. Biol. 19:797–802 with permission from Macmillan Publishers Ltd.; see that reference for more information.
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 5
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: A single gpD-YFP-labeled λ phage (green) diffusing near and on the Escherichia coli cell (red). Two examples are shown sequentially. For more information see Rothenberg et al. 2011 Biophys. J. 100:2875–82. Video courtesy of Eli Rothenberg Samuel Skinner and Ido Golding (unpublished).
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 6
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: DNA injection into the cell. The λ genome initially inside the capsid was labeled using a SYTOX Orange DNA dye. Video reproduced from Van Valen et al. 2010 Curr. Biol. 22:1339–43 with permission from Elsevier; see that reference for more information.
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 7
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: Spatiotemporal dynamics of phage (red) and bacterial (green) genome loci inside the infected Escherichia coli cell during the lysogenic pathway (see Figure 2b ). The phage genome is labeled using mCherry-ParB. In the same cell the bacterial attB site is detected using an orthogonal ParB system and a GFP. For more information see Tal et al. 2014 PNAS 111:7308–12. Video courtesy of Louis McLane and Samuel Skinner; Golding lab (unpublished).
Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 8
A video from the 2016 review by Ido Golding "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug" from the Annual Review of Virology.
Shown: Spatiotemporal dynamics of the phage genome inside the cell during the lytic pathway. At t = 0 min the cell is infected by a gpD-YFP-labeled phage (green). At t = 5 min a SeqA-ECFP focus corresponding to the injected phage DNA appears. Later this focus is converted to two foci corresponding to two hemimethylated phage DNAs. The cell eventually lyses (not shown). Video reproduced from Shao et al. 2015 Biophys. J. 108:2048–60 with permission from Elsevier.
More than Meets the Eye: Hidden Structures in the Proteome: Video 1
A video from the 2016 review by Hal Wasserman and Erica Ollmann Saphire "More than Meets the Eye: Hidden Structures in the Proteome" from the Annual Review of Virology.
Shown: Morphs modeling how the VP40 dimer might transition to the matrix-assembling linear hexamer (this video) and the RNA-binding octameric ring (Video 2). In each of these videos N-terminal domains are blue and C-terminal domains are orange. In this video construction of the hexamer form the dimer it is modeled that three VP40 dimers gather at the membrane surface. It is hypothesized that an electrostatic interaction with lipid head groups triggers conformational rearrangement and assembly of the hexamer. Note that in the crystal structure of this hexamer (PDB: 4LDD) the central VP40 is upside down. In Video 2 the crystal structure of the octameric ring (PDB: 1H2D; Gomis-Ruth et al. 2003 Structure 11:423–33) reveals a 3-nt RNA bound to each N-terminal domain. RNA is illustrated here as the trigger that begins the conformational rearrangement. However it is not yet known what the trigger of this rearrangement is. We thank Dr. Zachary Bornholdt for the use of these models and videos originally published as supplemental figures in Bornholdt et al. 2013 Cell 154:763–74.
More than Meets the Eye: Hidden Structures in the Proteome: Video 2
A video from the 2016 review by Hal Wasserman and Erica Ollmann Saphire "More than Meets the Eye: Hidden Structures in the Proteome" from the Annual Review of Virology.
Shown: Morphs modeling how the VP40 dimer might transition to the matrix-assembling linear hexamer (Video 1) and the RNA-binding octameric ring (this video). In each of these videos N-terminal domains are blue and C-terminal domains are orange. In Video 1 construction of the hexamer form the dimer it is modeled that three VP40 dimers gather at the membrane surface. It is hypothesized that an electrostatic interaction with lipid head groups triggers conformational rearrangement and assembly of the hexamer. Note that in the crystal structure of this hexamer (PDB: 4LDD) the central VP40 is upside down. In this video the crystal structure of the octameric ring (PDB: 1H2D; Gomis-Ruth et al. 2003 Structure 11:423–33) reveals a 3-nt RNA bound to each N-terminal domain. RNA is illustrated here as the trigger that begins the conformational rearrangement. However it is not yet known what the trigger of this rearrangement is. We thank Dr. Zachary Bornholdt for the use of these models and videos originally published as supplemental figures in Bornholdt et al. 2013 Cell 154:763–74.
More than Meets the Eye: Hidden Structures in the Proteome: Video 3
A video from the 2016 review by Hal Wasserman and Erica Ollmann Saphire "More than Meets the Eye: Hidden Structures in the Proteome" from the Annual Review of Virology.
Shown: A modeled morph between the two folds of lymphotactin. Residues 61–70 have been modeled into the final structure to facilitate the final morph and allow the complete α-helix to be included. The beginning structure is the originally disordered Ltn10 monomeric form that functions as a chemokine (PDB: 1J8I). The final structure is one protomer of the biologically active dimer Ltn40 which functions in chemotaxis (PDB: 1J8I).
The Magellanic Stream: Circumnavigating the Galaxy: Figure 4
A video from the 2016 review by Elena D'Onghia and Andrew J. Fox "The Magellanic Stream: Circumnavigating the Galaxy" from the Annual Review of Astronomy and Astrophysics.
Shown: Gas projection in the simulated Magellanic System (red scale). This model reproduces the length of the Magellanic Stream and Leading Arm as well as the Magellanic Bridge connecting the Small and Large Magellanic Clouds (SMC and LMC). In this animation the SMC has completed three passages around the LMC. Adapted from Besla et al. (2012) with permission.
Visual Adaptation: Supplemental Video 1
A supplemental video from the 2015 review by Michael A. Webster "Visual Adaptation" from the Annual Review of Vision Science.
Shown: Demonstrations of adaptation aftereffects described in the review. For each the same image is presented before or after 10 s of adaptation to an adapting image. The aftereffects refer to changes in the appearance of the image following the adaptation. Images illustrating color and blur aftereffects are courtesy of Menka Webster. Images illustrating face aftereffects are from Matsumoto D Ekman P. 1988. Japanese and Caucasian facial expressions of emotion (JACFEE) and neutral faces (JACneuf). Dep. Psychol. San Francisco State Univ.
Livestock's Lure and Liabilities
Herrero et al. "Livestock and the Environment: What Have We Learned in the Past Decade?" Annual Review of Environment and Resources (2015)
Patterns of Gun Violence in the United States
Wintemute. "The Epidemiology of Firearm Violence in the Twenty-First Century United States" Annual Review of Public Health (2015).
Data Visualization and Statistical Graphics in Big Data Analysis: Video 1
A video from the 2016 review by Dianne Cook Eun-Kyung Lee and Mahbubul Majumder "Data Visualization and Statistical Graphics in Big Data Analysis" from the Annual Review of Statistics and Its Application.
Shown: Still image of video showing plane movements across the United States on a normal day of operations January 19 2006. The video includes red-eye planes leaving the West Coast for the East Coast the East Coast waking up and sporadic delayed flights. The code (including links to the data) is available at https://github.com/heike/usflights. An accompanying video at https://vimeo.com/119233996 shows operations during a northeastern snow day March 13 1993. Used with the permission of Heike Hofmann.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 1
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 1 Unwinding of a single molecule of λ DNA by RecBCD.
This video shows unwinding of a single molecule of λ DNA – stained with YOYO-1 attached to a 1-μm polystyrene bead (stained nonspecifically by YOYO-1) and captured by an optical trap by a single RecBCD enzyme at 37°C in the presence of 1 mM ATP; DNA is extend by solution flow from the bottom to the top. This molecule unwound at ∼440 bp/sec and dissociated after ∼26800 bp. Published with permissions from Reference 30.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 2
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 2 Unwinding of a single molecule of DNA by RecBCD.
This video shows translocation of an individual RecBCD molecule along χ-containing λ DNA visualized by virtue of a fluorescent 40-nm particle attached to a biotinylated RecD subunit. Solution flow is left to right. The very bright spot to the left is the 1-μm polystyrene bead to which nanoparticles are bound nonspecifically in the optical trap. Note that the nanoparticle--RecBCD complex can be seen to pause for about 6.6 s and then it continues to translocate but at a much reduced speed (∼145 bp/sec versus ∼1120 bp/sec prior to χ-recognition). Published with permission from Reference 54.
Data Visualization and Statistical Graphics in Big Data Analysis: Video 2
A video from the 2016 review by Dianne Cook Eun-Kyung Lee and Mahbubul Majumder "Data Visualization and Statistical Graphics in Big Data Analysis" from the Annual Review of Statistics and Its Application.
Shown: Use of interactive graphics to explore rankings of statistics departments in the United States. The plots show rating variables as side-by-side dotplots (left) a cluster analysis (center top) and a scatterplot of 5th-percentile rank computed using the S (vertical) and R (horizontal) methods (center bottom) and institution name lookup (right). Selecting an institution highlights (yellow) its values in each of the other plots. Cornell University is highlighted: We can see that its rank by the two methods differs substantially with a good R rank (around 5) but not such a good S rank (around 30). On the ranking criteria the department is around the middle of the pack: It is average in terms of number of publications and citations has few women faculty and students and accepts students with lower GRE scores than most statistics departments.
Data Visualization and Statistical Graphics in Big Data Analysis: Figure 9
A video from the 2016 review by Dianne Cook Eun-Kyung Lee and Mahbubul Majumder "Data Visualization and Statistical Graphics in Big Data Analysis" from the Annual Review of Statistics and Its Application.
Shown: Eulerian parallel coordinate plots (PCPs) of nutritional measurements of chocolates. The Eulerian PCP more strongly indicates a difference between the two types of chocolate milk (orange) and dark (red) and from the scagnostic describing separation displayed by the histogram (blue) we learn that higher values occur when fiber is one of the two variables indicating its importance as a variable. A simple interaction added to this plot using gridSVG highlights the line closest to the mouse cursor so that we can follow it throughout the plot.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 3
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 3 Interrupting and restarting single molecules of RecBCD during DNA unwinding.
This video compares unwinding of three individual molecules of λ DNA by three different single molecules of RecBCD whose initial velocities are comparable. Solution flow is left to right. For each the bright spot to the left is the 1-μm polystyrene bead to which YOYO-1 is bound nonspecifically in the optical trap. Unwinding is transiently paused by moving the molecules into a solution of EDTA (denoted by “Paused” during the video) and then resumed by returning the molecules to a solution of Mg2+:ATP. The change in relative unwinding rate is visually evident. Videos are representative of data collected and reported in Reference 31 although the video itself is previously unpublished.
Ocean Basin Evolution and Global-Scale Plate Reorganization Events Since Pangea Breakup: Supplemental Video 1
A supplemental video from the 2016 review by R. Dietmar Müller Maria Seton Sabin Zahirovic Simon E. Williams Kara J. Matthews Nicky M. Wright Grace E. Shephard Kayla T. Maloney Nicholas Barnett-Moore Maral Hosseinpour Dan J. Bower and John Cannon "Ocean Basin Evolution and Global-Scale Plate Reorganization Events Since Pangea Breakup" from the Annual Review of Earth and Planetary Sciences.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 4
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 4 Unwinding of a single molecule of λ DNA by RecQ detected using fluorescent SSB and imaged using TIRF microscopy.
Initially the dsDNA tethered at each end to the surface of a flow cell is fluorescent due to intercalation of the YO-PRO-1 dye molecules. As a solution containing 200 mM NaCl is introduced into the flow cell the dye dissociates and fluorescence disappears. Subsequently a solution containing 80 nM RecQ and SSBAF488 is introduced into the flow cell to initiate unwinding. The solution fills the channel 24 s into the video. The flow when on goes from left to right in the video. The elapsed time is indicated in hours:minutes:seconds and scale bar is 5 μm. Published with permission from Reference 73.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 5
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 5 Salt-induced intramolecular condensation of SSB-ssDNA imaged using TIRF microscopy.
Video of a single molecule of SSBAF488-coated λ ssDNA tethered at one end and imaged using TIRF microscopy contracting in length upon increasing [NaOAc] from 0 to 100 mM. Solution flow is left to right. The video frames were rendered into a topological intensity map. Time zero corresponds to the time at which the pump was turned on. The dead time of the experiment was approximately 25 s due to the volume in the lines between the syringe valve and the microfluidic chamber. Published with permission from Reference 102.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 6
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 6 Condensation of SSB in the absence of free protein during a transient increase from 0 to 100 mM NaOAc.
Video of a single molecule of SSBAF488-coated λ ssDNA contracting in length as the salt concentration is increased from 0 to 100 mM NaOAc and then subsequently reduced back to zero mM conducted in the absence of free SSBAF488. Solution flow is left to right. The flow cell was extensively washed with buffer to remove free SSB protein before beginning the experiment. Video recording began when the pump was turned on requiring ∼40-50 s for the dead volume to be flushed from the lines to the flow chamber. SSBAF488 was omitted from both of the high-salt washes and from the 0 mM wash. Published with permission from Reference 102.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 7
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 7 Direct imaging of nucleation and growth of RecA on SSB-coated ssDNA using TIRF microscopy.
This video first shows a flow-extended single molecule of 3′-biotinylated λ ssDNA coated with a fluorescent protein SSBAF488. The molecule was tethered to a streptavidin-coated glass surface within a microfluidic flow cell and visualized using total internal reflection fluorescence microscopy. Solution flow is left to right. SSBAF488 was exchanged with non-fluorescent SSB on the ssDNA in situ and filament assembly was initiated by injecting fluorescein labeled RecA (350 nM) with nucleotide cofactor (ATPγS). Filament assembly proceeded primarily in the absence of flow or laser excitation which were both used only during brief intermittent time-lapsed imaging. Images were processed and rendered into a topographical intensity map for clarity. Published with permission from Reference 110.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 8
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 8 Optical trapping and manipulation of single molecules of gapped λ DNA for direct imaging of RecA filament assembly.
This video first shows 1-μm streptavidin-coated polystyrene beads flowing through Channel 1 of a multichannel microfluidic flow chamber and the subsequent isolation of two beads by a split-beam dual optical trap (Step 1). Solution flow is left to right. The beads are then transferred to Channel 2 which contains gapped λ DNA molecules comprising 8155 nucleotides of SSB-coated ssDNA flanked by 21.08 and 24.59 kbp of YOYO-1 stained dsDNA; the gapped DNA is biotinylated at each of the molecule and is captured in situ by binding to the streptavidin-coated beads (Step 2). The molecule is then transferred to a DNA-free Channel 3 where the distal end of the flow-extended molecule is captured by the other bead which is micromanipulated using a steerable mirror in line with one of the infrared laser beams (Step 3). The molecule is then rotated perpendicular to flow and imaged in buffer optimized for visualizing YOYO-1 stained dsDNA in Channel 4 (Step 4). The molecule is then transferred to Channel 5 containing Mg2+:ATPγS which accelerates YOYO-1 dissociation (Step 6). The molecule was then successively incubated in reaction buffer containing fluorescein labeled RecA Mg2+:ATPγS and either RecOR or RecFOR and imaged in Channel 5 to measure the rates of nucleation and growth (Step 7). Published with permission from Reference 110.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 9
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 9 Composite video depicting the experimental procedure used to visualize DNA pairing on single λ DNA-dumbbell molecules by optical trapping.
A DNA pairing reaction (2 min) was performed with the 430 nt substrate at a 2 μm bead distance. Text and illustrations were inserted at appropriate places to facilitate description. A four-channel flow cell with a flow-free reservoir was used. Solution flow is top to bottom. First two 1-μm streptavidin-coated polystyrene beads are captured in dual optical traps. Next a single DNA molecule is captured on one bead. The DNA-dumbbell is made by sliding the DNA along the other bead until the biotinylated end attaches. The DNA end-to-end distance is set and the YOYO-1 dye is removed. The DNA-dumbbell is incubated with fluorescent RecA nucleoprotein filaments in the flow-free reservoir for 2 min. Afterward the DNA-dumbbell is moved to the observation channel and is extended to near contour length to image the pairing products. Published with permission from Reference 152.
Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 10
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski "Mechanics and Single-Molecule Interrogation of DNA Recombination" from the Annual Review of Biochemistry.
Supplemental Video 10 Dissociation of heterologously paired RecA nucleoprotein filaments during a DNA pairing experiment.
Video performed as in Supplemental Video 9 showing RecA nucleoprotein filaments both heterologously bound and homologously bound (left and right red spots respectively) during the extension step of a pairing assay performed using the 1762 nt homologous ssDNA. As the beads are separated several loop-release events are observed involving the heterologously bound filament (left) before its dissociation from λ DNA whereas the homologously bound RecA nucleoprotein filament (right) remains stably bound. Published with permission from Reference 152.
Leading Leadership Research: A Framework for Research and Practice
David Day of The University of Western Australia Business School discusses his article "Leadership Development: An Outcome-Oriented Review Based on Time and Levels of Analyses." In this video he outlines a framework for leader development over time. Dr. Day explains that focusing research and intervention on proximal indicators like competencies and self-views helps determine longer-term outcomes such as hierarchical complexity and sophisticated sense-making matching these to the leaders’ environments.
Developing Your Leaders: Linking Short-Term Change to Long-Term Success
David Day of The University of Western Australia Business School discusses his article "Leadership Development: An Outcome-Oriented Review Based on Time and Levels of Analyses." In this video he outlines a framework for leader development over time. Dr. Day explains that focusing research and intervention on proximal indicators like competencies and self-views helps determine longer-term outcomes such as hierarchical complexity and sophisticated sense-making matching these to the leaders’ environments.
Lessons for Leadership Scholars: Where Can You Take Your Research?
What Can Leadership Development Do for Your Organization?
Lisa Dragoni of Wake Forest University discusses her article "Leadership Development: An Outcome-Oriented Review Based on Time and Levels of Analyses” which she co-wrote with David Day of the University of Western Australia Business School. In this video she reviews the research on leadership development. Dr. Dragoni outlines the conditions that support leadership development at an organizational level. These include interpersonal comfort among team members their expertise and shared mindset.
The Growing Impact of Citizen Astronomers
Marshall et al. "Ideas for Citizen Science in Astronomy"
Insect Flight: From Newton's Law to Neurons: Video 1
A video from the 2016 review by Z. Jane Wang "Insect Flight: From Newton's Law to Neurons" from the Annual Review of Condensed Matter Physics.
Shown: A model fly hovers briefly and succumbs to pitching instability. This is an example of flight instability with feedback control (1; also see Section 2).
Insect Flight: From Newton's Law to Neurons: Video 2
A video from the 2016 review by Z. Jane Wang "Insect Flight: From Newton's Law to Neurons" from the Annual Review of Condensed Matter Physics.
Shown: With a time-delayed discrete feedback control scheme the model fly can hover stably (1; also see Section 3.4).