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Annual Review of Biophysics

Volume 48, 2019

Giant Vesicles and Their Use in Assays for Assessing Membrane Phase State, Curvature, Mechanics, and Electrical Properties

Abstract

Giant unilamellar vesicles represent a promising and extremely useful model biomembrane system for systematic measurements of mechanical, thermodynamic, electrical, and rheological properties of lipid bilayers as a function of membrane composition, surrounding media, and temperature. The most important advantage of giant vesicles over other model membrane systems is that the membrane responses to external factors such as ions, (macro)molecules, hydrodynamic flows, or electromagnetic fields can be directly observed under the microscope. Here, we briefly review approaches for giant vesicle preparation and describe several assays used for deducing the membrane phase state and measuring a number of material properties, with further emphasis on membrane reshaping and curvature.

Keyword(s): electroporation, membrane domains, membrane elasticity, membrane pores, membrane tubes, microcompartments, model membranes, phase diagrams, spontaneous curvature
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/content/journals/10.1146/annurev-biophys-052118-115342
2019-05-06
2024-04-26
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/content/journals/10.1146/annurev-biophys-052118-115342
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Giant Vesicles and Their Use in Assays for Assessing Membrane Phase State, Curvature, Mechanics, and Electrical Properties
Annual Review of Biophysics 48, 93 (2019); https://doi.org/10.1146/annurev-biophys-052118-115342
/content/journals/10.1146/annurev-biophys-052118-115342
/content/journals/10.1146/annurev-biophys-052118-115342
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