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In eukaryotic cells, the spatial segregation of replication and transcription in the nucleus and translation in the cytoplasm imposes the requirement of transporting thousands of macromolecules between these two compartments. Nuclear pore complexes (NPCs) are the sole gateways that facilitate this macromolecular exchange across the nuclear envelope with the help of soluble transport receptors. Whereas the mobile transport machinery is reasonably well understood at the atomic level, a commensurate structural characterization of the NPC has only begun in the past few years. Here, we describe the recent progress toward the elucidation of the atomic structure of the NPC, highlight emerging concepts of its underlying architecture, and discuss key outstanding questions and challenges. The applied structure determination as well as the described design principles of the NPC may serve as paradigms for other macromolecular assemblies.
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Supplemental Movie 1: Overall, the nuclear pore complex (NPC) consists of a cylindrical, symmetric core, which is asymmetrically decorated with filaments and a nuclear basket structure on the cytoplasmic and nucleoplasmic sides, respectively. Molecules smaller than ∼40 kDa (small spheres) freely diffuse through the NPC, whereas larger noncargo molecules (large spheres) are prevented from crossing the nuclear envelope. Artwork by Joseph Alexander, Erik W. Debler, and André Hoelz. Download movie file (MOV)
Supplemental Movie 2: Active import and export of cargoes are facilitated by nuclear localization and nuclear export sequences (NLS and NES, respectively) that are recognized by transport factors, collectively termed karyopherins (Kaps). The NLS of import cargoes (blue) is recognized either directly by an import karyopherin-β (Kap-β; salmon) or via an adapter karyopherin (Kap-α; light green). RanGTP (red) binding inside the nucleus leads to dissociation of the import complex. By contrast, the assembly of a NES-cargo Kap-β export complex requires RanGTP binding (represented in blue, yellow, and red, respectively). In the cytosol, this export complex is dissociated by GTP hydrolysis, which is catalyzed by Ran GTPase-activating protein (RanGAP; dark green) or Ran-binding protein 1 (RanBP1). Artwork by Joseph Alexander, Erik W. Debler, and André Hoelz. Download movie file (MOV)
Supplemental Movie 3: The transport of large cargoes (blue) is thought to require the dilation of the central channel of the NPC. Artwork by Joseph Alexander, Erik W. Debler, and André Hoelz. Download movie file (MOV)
Supplemental Movie 4: Inner nuclear membrane (INM) proteins are cotranslationally integrated into the endoplasmic reticulum membrane, which is continuous with the outer nuclear membrane, and then imported to the INM. Similar to the karyopherin-mediated transport in Movie 2, the transport of INM proteins is also dependent on the Ran cycle and karyopherins that likely travel through the central channel, whereas the cargo protein is anchored in the membrane. INM proteins, Kap-α, Kap-β, and RanGTP, are illustrated in light purple, light green, brown, and red, respectively. Substantial structural changes within the NPC would be necessary to facilitate this transport event. Artwork by Joseph Alexander, Erik W. Debler, and André Hoelz. Download movie file (MOV)